XplaceH2 | Serum replacement, human cell line-derived

XplaceH2 is a supplement for serum-free cell culture processes. It contains proteins produced by continuously growing hepatocyte-derived cell lines in a concentrated and purified form.
The supplement is available in two variations:

• As a frozen liquid (100 mg | 10 mg/ml)  (108101-100MG).
• As a lyophilised solid (10 mg)  (108102-10MG), which provides improved shipping and storage behaviour and is reconstituted in sterile water

It is used to replace foetal calf serum as well as human serum or platelet lysate from standard media for cell cultivation and contains proteins such as:

• Transportproteins: Transferrin, serum albumin (low amount), transthyretin, apolipoprotein B-100
• Adhesion factors: Fibronectin, various laminins
• Protease inhibitors: alpha-1-antitrypsin, alpha-2-macroglobulin, plasminogen activator inhibitor
• low amount in growth factors

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Application note | Characteristics

XplaceH2 is sterile and ready-to-use upon reconstitution or thawing, respectively. Since XplaceH2 is produced in a controlled, chemically defined process as well as dialysed, small molecules such as hormones, metabolites and media components are not present.

It can be applied to cell cultures directly at a concentration of 5 - 100 mg/L final volume.

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