SciFY10 | Chemically defined feed medium
CHF 69.00 – CHF 89.00
SciFY10 was been developed to improve the growth and productivity in mammalian cell-based fed batch processes in suspension culture. The chemically defined, protein-free formulation provides essential as well as beneficial nutrients.
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Characteristics
SciFY10 is available in different formates:
- w/o L-glutamine, w/o D-(+)-glucose
- w/o L-glutamine, with D-(+)-glucose
- with L-glutamine, with D-(+)-glucose
SciFY10 contains neither phenol red, antibiotics nor synthetic polymers such as Poloxamer 188.
On request, variations in media formulation can be performed.
The medium is provided as a 0.22 μm sterile filtered as well as sterility tested liquid. The liquid medium is free of mycoplasma.
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Application note
The feed medium SciFY10 was optimised to be applied daily at 5 % (v/v) starting from day 3.
Optimally, the L-glutamine and D-(+)-glucose concentrations should be monitored throughout the process and be adjusted to 1-2 mM or 1-2 g/L, respectively.
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Compatible cell lines
- CHO-K1
- CHO-DG44
- ...and surely more, but were not tested yet
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