CHOsulu reporter cell lines | serum-free detection of hormonal activity with suspension cells

Next to all the animal-free products, Scinora has developed serum-free reporter cell lines specifically designed to fit in automated systems for screening as well as monitoring. Our CHOsulu series of reporter cell lines in suspension provides a convenient and versatile workflow with fast detection below 24 hours.

All CHOsulu reporter cell lines are based on CHO-K1 adapted to a spezialised chemically defined, serum-free medium. CHOsulu reporter cell lines encode the secreted Gaussia luciferase under the control of the respective soluble human receptor and response element. The secreted Gaussia luciferase enabels a constant detection starting at 14 hours after treatment.

Due to the growth in susupension, the CHOsulu reporter cell lines can be used in polypropylene (PP) storage plates used in library screenings due to their solvent resistence. Standard tissue culture polystyrene or glass plates can be used as well.

Currently, the following cell lines with the respective human receptor are available:
– CHOsulu-ERalpha (estrogen receptor alpha)
– CHOsulu-ERbeta (estrogen receptor beta)
– CHOsulu-GR (glucocorticoid receptor)
– CHOsulu-TRbeta (thyroid receptor beta)

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Application note | Characteristics

CHOsulu reporter cell lines are available as frozen ready-to-use aliquots to immediately use the cells after thawing and prior hastle-free sample preparation:

Each CHOsulu reporter cell line kit for 10x 96-well plates includes:

• 10x frozen aliquots, ready-to-use, containing 10e7 cells each
• 125 ml CHOsulu assay medium, serum-free, protein-free and chemically defined
• 125 ml development buffer for luminescence detection for simple addition after incubation.

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Potential applications

• Environmental sample fractionation with subsequent effect-based detection
• Library screen
• Risk assessment
• Quality control

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Additional information