CultS9R | cultivated, rat liver cell line-derived S9 fraction

CultS9R is a first of its kind in vitro metabolisation agent derived from a chemically defined, animal component-free production process using rat liver-derived suspension cell cultures. It replaces the commonly used pooled rat liver S9 fraction and can be used as alternative to the rat liver S9 fraction as well.

The supplement is available as a lyophilised aliquote with 20 mg protein (109106-20MG) and each batch is characterised as following:

__________________________________________________________________________________________________________

Application note | Characteristics

CultS9R is sterile and ready-to-use upon reconstitution with sterile water, respectively. This metabolic agent can by applied such as standard rat S9 or its animal variants. The optimised concentration is 0.25 mg/ml within your buffered cofactor mix consiting of NADP, glucose-6-phosphate and magnesium salts. The enzyme activity for NADPH regeneration is sufficient for standard applications. Higher concentrations are possible and may ensure compatibility with established protocols.

Since it is sterility tested and free of mycoplasma, CultS9R can be applied to serum-free as well as FCS-containing cell cultures directly at a concentration of 50 - 500 mg/L final volume.

_________________________________________________________________________________________________________

Protocol

Standard metabolisation

• Dissolve your compounds in sodium phosphate buffer (pH 7.4; 100 mM);
• Add the same volume of a mixture of 0.5 mg/ml CultS9R in sodium phosphate buffer (pH 7.4; 100 mM) containing 6 mM NADP, 30 mM glucose-6-phosphate and 10 mM magnesium chloride;
• Incubate the mixture for 30-120 minutes at 33 - 37 °C under constant shaking.